INFERTILITY CONSULTATION
In today’s times, due to increase in the incidences of late marriages, stress levels, pollution etc. infertility seems to be ever growing problem faced by many couples all over the world.
Infertility is not a disease and can be faced by any couple irrespective of their age, caste or socio-economic status. Therefore there is a need for awareness amongst couples.
40% of the infertile couples have problems in the female partner, 40% have male factor infertility, 10% couples have problems in both the partners while 10% couples fall into the category of unexplained infertility.
At Ruby Hall Clinic’s IVF & Endoscopy Center, a detailed history of the couples is documented, followed by the accurate diagnosis and the couple is then informed about the know-how of the exact treatment option (s) available. Counseling is also available for those couples needing the service.

GYNECOLOGICAL CHECK UP
General medical and surgical care for women's diseases, especially relating to their reproductive organs is offered at Ruby Hall Clinic’s IVF & Endoscopy Center.

ANDROLOGY
Andrology - is the medical specialty that deals with men's fertility, particularly relating to the problems of the male reproductive system and treatment of male infertility.
We perform Semen Analysis based on the WHO criteria. The sperms are magnified 7200 times and analysed strigently for the morphology in teratozoospermia.
Our center also runs a sperm bank wherein husband’s sperm can be frozen in special cases.

HORMONAL ASSAYS
Reproductive hormone tests (female) and comprehensive semen analysis (male).

ULTRASOUND
Obstetrical Ultrasound Services
Ultrasonography has proved invaluable for monitoring pregnancy in a variety of ways, including identification of foetal anomalies, detection of the baby's position and placental location and determination of the baby's sex.
Ultrasonography procedures include:
Assessment and Evaluation
First trimester evaluation
Second and third trimester anatomical assessment
Prenatal diagnosis
Screening
First and Second trimester Down syndrome screening
Miscarriage and ectopic pregnancy screening
Multiple pregnancy screening
Foetal anomaly screening
Gynaecological Ultrasound Services
Applications of Gynaecological ultrasound include general screening of women for:
Ovarian, uterine, and tubal pathology evaluation
Follicular monitoring
Sonohysterography

DRUG THERAPY
It is recommended for Ovulation Induction, Infertility Diseases, Hormone Treatment & Regulation, HRT
Ovulation Induction - The use of medication to promote ovulation in women who normally do not ovulate or ovulate very irregularly.
Infertility Diseases - Drug treatment for various types of diseases causing infertility.
Hormone Treatment & Regulation - Using hormonal drugs as part of the regime to correct infertility.
HRT - Advice on use of Hormone replacement therapy (HRT).

FERTILITY ENHANCING ENDOSCOPIC SURGERIES
Microsurgery / Minimal Invasive /
Laparoscopic (Key-Hole) Surgery
Bilateral Tuboplasty - The surgical repair of both damaged fallopian tubes to treat infertility.
Laparoscopic Varicocoelectomy - Using keyhole surgery to ligate the varicose veins that drain blood from the testes to improve sperm quality for men associated with varicocoeles and poor semen quality.
PESA (Percutaneous Epididymal Sperm Aspiration) - This is a non-invasive method of sperm recovery and has excellent potential for patients who have had a vasectomy and do not wish to undergo reversal, or patients who have had an attempted reversal which has failed. In percutaneous epididymal sperm aspiration sperm are aspirated through a fine needle that is placed into the epididymis.
TESA (Testicular Sperm Aspiration) - This is a surgical sperm retrieval procedure used in fertility treatment for men who have no sperm in their ejaculate. The extraction of sperm is achieved through a fine needle inserted into the testes. Where this is not successful, then a microsurgical testicular biopsy is done.
Testicular Biopsy - The removal of small testicular tissue from men with very poor sperm production and the isolation of sperm for ICSI.
Vas Epididymis Exploration - Exploratory surgery to check for blockage of sperm flow.

ARTIFICIAL INSEMINATION (AI)
Artificial insemination is just one of the options that may be considered prior to attempting more involved treatments, such as IVF. AI is a relatively simple procedure that involves injecting a sample of specially treated sperm from the husband into the wife's reproductive tract. The AI is scheduled around the same time when the wife is expected to ovulate.
The semen is washed free of debris, other cells and dead and abnormal sperms before it is transferred in the uterus.
Since the washed sperms are released into the uterus, this procedure is also referred as IUI (Intra-Uterine Insemination).
IUI has been used to treat infertility for many years and is most often employed where there is mild male factor infertility, antigen/antibody reactions in the cervical mucus, or a male donor. Success rates with IUI are dependent upon many factors including the age of the female, the quality and quantity of the sperm and the causes(s) of infertility. Follicle stimulating hormone or Clomid are often used in stimulated IUI.


In Vitro Fertilization (IVF)
IVF involves stimulation of the female partner to produce more number of eggs at a time. When majority of these eggs mature, Ovum pick-up (OPU) is scheduled. OPU is a day care procedure which is done under general anesthesia. The eggs retrieved are cultured for 3-4 hours before proceeding with IVF washed sperms. Calculated number of sperms are released in the vicinity of the eggs and fertilization occurs naturally with one sperm entering the egg to form an embryo.


In Cytoplasmic Sperm Injection (ICSI)
ICSI is a highly specialized laboratory procedure developed to help infertile couples due to male factor infertility where there are abnormalities in the number, quality, or function of the sperm.
The term 'Intracytoplasmic Sperm Injection" refers to the direct injection of a single sperm into a mature egg of the female. The sperms are magnified 400 times and selected based on their normal morphology before injecting in the egg.
Today, ICSI is the method of choice except in cases where the egg quality of the female is extremely poor and in cases of unexplained infertility.


IMSI- Intra-cytoplasmic Morphologically selected Sperm
Injection is the latest technology in the field of Assisted Reproductive Techniques.
Ruby Hall IVF & Endoscopy Center, Pune is one of the very few ART centers all over the world to be equipped to perform IMSI.
Currently, the sperm is magnified only 400 times. IMSI enables us to magnify the sperm as high as 7200 times. This high magnification allows
the embryologists to select a morphologically normal sperm. IMSI thereby improves the pregnancy outcomes and reduces abortion rates.


Cryopreservation
The eggs, sperms and embryos can be cryopreserved in the liquid nitrogen for future use.
Sperm Cryopreservation is done in cases where the husband is unavailable during the wife’s treatment, or in patients with severe male factor infertility, or in cancer patients.
Egg Cryopreservation is recommended in young patients with cancer. It is also routinely done in the countries where there are legal limitations in the number of embryos to be developed.
Embryo Cryopreservation is done in the cases where there are good quality surplus embryos. The patients who are hyper stimulated, embryo Cryopreservation helps to avoid further complications. It is also useful wherein the embryo transfer is to be postponed in emergency cases.

Extended Culture
A blastocyst is an embryo that has developed in culture for at least five days after fertilization and then is transferred in the female womb. This is a more physiological approach since it is known that under in-vivo conditions the embryos reach the uterine cavity around the end of the sixth day following fertilization.
This also allows at times for natural selection of best quality embryo as only the better quality, genetically normal embryo will grow to this stage.

Embryo Transfer
Embryo transfer is a process wherein the in vitro cultured embryos are replaced back in to the uterus to establish pregnancy. The embryos are transferred on day 3 (Cleavage stage embryo) or day 5 (Blastocyst stage embryo) of the culture period. Embryo transfer can be done either by using fresh eggs of the same menstrual cycle or by using cryopreserved eggs from previous menstrual cycle undergone freezing. The transfer is usually carried out under ultrasound guidance by using a soft catheter loaded with embryos.
Day 1, Day 2, Day 3, Day 5

Donor Programme
Sometimes, the couple may have to resort to the donor programme (either the eggs, sperms or the embryos) as the best possible option for conception. This treatment is advised to the couples wherein one or both the partners have a higher probability of transmitting a genetic disorder to their children or in cases where the sperms or eggs produced are extremely low in quality.

Donor Egg
Donor egg is an option for couples whose eggs will no longer fertilize and produce healthy embryos. Egg quality generally declines with advancing female age but it can occur at any age. Ovarian reserve is a measure of "egg quality" and is evaluated during the infertility workup.
Other candidates for donor egg include women who may have been born with absent ovaries (rare) or those whose ovaries were damaged by cancer chemotherapy or radiation. Donor eggs may also be used in cases of unexplained infertility and severe endometriosis

Donor sperms:
The couple may opt for the donor sperm when the male partner is sterile (testicular failure) and therefore unable to provide a sperm sample for IVF or ICSI.
In other instances, the couple may look to use a sperm donor in order to avoid passing on a genetic disease or disorder that is carried by the male sperm.

Donor Embryo
When both couples are faced with infertility problems, embryo donation can be an excellent choice. Issues that typically cause a couple to consider embryo donation include:
- Poor sperm production and count
- Problems with egg development and ovulation
- Attempts at other types of ART have been unsuccessful
- High risk of passing on genetic disorders but are unable to pursue PGD
- Other fertility treatments are not financially feasible

Surrogacy
Surrogacy can easily be defined as the process whereby another woman carries the child of infertile couple to term. Upon birth, the child is handed over to the couple, with the gestational carrier giving up any legal rights she may have to the child. There are a variety of reasons why a couple may choose to use a surrogate carrier. Women who have absent uterus, diseased uterus or surgically removed for any other cause are advised for surrogacy. Even the women with medical diseases like heart or renal disease who are unable to take the load of pregnancy can go for surrogacy.
